Volume 25, Issue 4 (July 2017)                   JSSU 2017, 25(4): 264-270 | Back to browse issues page

XML Persian Abstract Print

Abstract:   (5020 Views)
Introduction: Delay in wound healing is one of several complications of type 1 diabetes. It has been reported that the use of various herbal extracts such as Morus nigra increase the  wound healing.  Since,there are no studies on investigation of the effects of Morus nigra on wound healing in diabetics so this study was conducted to determine wound healing effects of Morus nigra in diabetic rats.
Methods: In this experimental study, the fresh fruits were blended and pressed. Then, the extract was filtered and then was passed through the filter paper then it was dried in shade. Diabetes was induced by injection of single dose of streptozotocin (60 mg/kg). The circular wound (diameter 8 cm) was created on the back of each rat. The wounds were topically treated with 10 or 20 percent of concentration of the extract. Wounds in control group were treated with saline and normal group received no treatment.  Wound areas were monitored on days 1, 6 and 11; the repaired tissues were removed for histological evaluations.
Result: Daily and local applications of the extract displayed significant wound healing activity through the reducing wound area and acceleration of wound contraction. Histological findings showed that various indices of tissue repairment such as  integrated epithelial,  typical granulated and regular collagen layers were mor obvious in  the treated wounds.
Conclusion: Topical application of the Morus nigra extract can accelrate wound contraction and improve the histological indices inclue formation of epithelial and granulated layers and increase collagen density in the repaired tissue.
Full-Text [PDF 598 kb]   (1250 Downloads)    
Type of Study: Original article | Subject: Physiology
Received: 2016/09/5 | Accepted: 2016/11/26 | Published: 2017/09/3

1. Komesu MC, Tanga MB, Buttros KR, Nakao C. Effects of acute diabetes on rat cutaneous wound healing. Pathophysiology 2004; 11(2): 63-7.
2. Sullivan KA, Hayes JM, Wiggin TD, Backus C, Oh SS, Lentz SI, et al. Mouse models of diabetic neuropathy. Neurobiology of disease 2007; 28(3): 276-85.
3. Jeffcoate WJ, Harding KG. Diabetic foot ulcers. Lancet 2003; 361: 1545.
4. Schäffer MR, Tantry U, Efron PA, Ahrendt GM, Thornton FJ, Barbul A. Diabetes-impaired healing and reduced wound nitric oxide synthesis: a possible pathophysiologic correlation. Surgery 1997; 121(5): 513-9.
5. Ercisli S, Orhan E. Chemical composition of white (Morus alba), red (Morus rubra) and black (Morus nigra) mulberry fruits. Food Chem 2007; 103(4): 1380-4.
6. Bang IS, Park HY, Yuh CS, Kim AJ, Yu CY, Ghimire B, et al. Antioxidant activities and phenolic compounds composition of extracts from mulberry (Morus alba L.) fruit. Korean Med Crop Sci 2007; 15(2): 120-7.
7. Bang IS, Park HY, Yuh CS, Kim AJ, Yu CY, Ghimire B. et al. Antioxidant activities and phenolic compounds composition of extracts from mulberry (Morus alba L.) fruit. Korean J Med Crop Sci 2007; 15(2): 120-7.
8. Kim YC, Kim MY, Takaya Y, Niwa M, Chung SK. Phenolic antioxidants isolated from Mulberry leaves. Food Sci and Biotech 2007; 16(5): 854-7.
9. Uzochukwu IC, Osadebe PO. Comparative evaluation of antidiabetic activities of Flavonoids extract and crude methanol extract of loranthus Micranthus parasitic on. J Pharmaceutical and Allied Sciences 2007; 4(1): 2-7.
10. Rice-evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridham JB. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free radical research 1995; 22(4): 375-83.
11. Kaul TN, Middleton E, Ogra PL. Antiviral effect of flavonoids on human viruses. J med virology 1985; 15(1): 71-9.
12. Sak K, Everaus H. Role of flavonoids in future anticancer therapy by eliminating the cancer stem cells. Current stem cell research & therapy 2015; 10(3): 271-82.
13. Andallu B, Suryakantham V, Srikanthi BL, Reddy GK. Effect of mulberry (Morus indica L.) therapy on plasma and erythrocyte membrane lipids in patients with type 2 diabetes. Clinica Chimica Acta 2001; 314(1): 47-53.
14. Andallu B, Varadacharyulu NC. Control of hyperglycemia and retardation of cataract by mulberry (Morus indica L.) leaves in streptozotocin diabetic rats. Indian j experimental biology 2002; 40(7): 791-5.
15. Andallu B, Kumar AV, Varadacharyulu N. Lipid abnormalities in streptozotocin-diabetes: Amelioration by Morus indica L. cv Suguna leaves. International j diabetes in developing countries 2009; 29(3): 123-28.
16. Nakamura S, Hashiguchi M, Yamaguchi Y, Oku T. Hypoglycemic effects of Morus alba leaf extract on postprandial glucose and insulin levels in patients with type 2 diabetes treated with sulfonylurea hypoglycemic agents. Journal of Diabetes & Metabolism 2011; 2(9): 158.
17. Nikkhah E, Khayami M, Heidari R. In vitro screening for antioxidant activity and cancer suppressive effect of Blackberry (Morus nigra). Iranian j cancer prevention 2008; 1(4): 167-72.
18. Singer AJ, Clark RA. Cutaneous wound healing. New England journal of medicine 1999 2; 341(10): 738-46.
19. Bielefeld KA, Amini-Nik S, Alman BA. Cutaneous wound healing: recruiting developmental pathways for regeneration. Cellular and Molecular Life Sciences 2013; 70(12): 2059-81.
20. Brem H, Tomic-Canic M. Cellular and molecular basis of wound healing in diabetes. The J clinical investigation 2007; 117(5): 1219-22.
21. Akkol, Esra Küpeli, et al. "Bioassay-Guided Isolation and Characterization of Wound Healer Compounds from Morus nigra L.(Moraceae)." gene expression 10 (2015): 11. (2015): 484-495.
22. Stintzing FC, Stintzing AS, Carle R, Frei B, Wrolstad RE. Color and antioxidant properties of cyanidin-based anthocyanin pigments. J Agricultural and Food Chemistry 2002 9; 50(21): 6172-81.
23. Özgen M, Serçe S, Kaya C. Phytochemical and antioxidant properties of anthocyanin-rich Morus nigra and Morus rubra fruits. Scientia Horticulturae 2009 3;119(3):275-9.
24. Naderi, Gholam Ali, et al. "Antioxidant activity of three extracts of Morus nigra." Phytotherapy Research 18.5 (2004): 365-369.
25. Özgen, Mustafa, Sedat Serçe, and Cemal Kaya. "Phytochemical and antioxidant properties of anthocyanin-rich Morus nigra and Morus rubra fruits." Scientia Horticulturae 119.3 (2009): 275-279.

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.