Comparison of Genomic Polymorphisms and Genetic Relation of Clinical Salmonella Enterica Serovar Typhimurium Isolates in Kerman Province by ERIC- PCR and Box-PCR Methods

Geranmayeh, Arghavan; Hoseini, Farzaneh; Rafiei Tabatabaei, Robab

Volume 25, Issue 7 (oct 2017) JSSU 2017, 25(7): 564-571 | Back to browse issues page

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Geranmayeh A, Hoseini F, Rafiei Tabatabaei R. Comparison of Genomic Polymorphisms and Genetic Relation of Clinical Salmonella Enterica Serovar Typhimurium Isolates in Kerman Province by ERIC- PCR and Box-PCR Methods . JSSU 2017; 25 (7) :564-571
URL: http://jssu.ssu.ac.ir/article-1-3765-en.html

Comparison of Genomic Polymorphisms and Genetic Relation of Clinical Salmonella Enterica Serovar Typhimurium Isolates in Kerman Province by ERIC- PCR and Box-PCR Methods

Arghavan Geranmayeh

, Farzaneh Hoseini ^*

, Robab Rafiei Tabatabaei

Abstract: (4018 Views)

Introduction: Salmonella is one of the most important causes of gastroenteritis in humans. Salmonella enterica Serovar Typhimurium has many hosts in addition to humans, and its prevalence in the community is high. The aim of the study was comparing the genetic diversity of Salmonella enterica serovar Typhimurium isolated from human fecal samples by both of ERIC-PCR and BOX-PCR method.
Methods: In this cross-sectional study, 60 Salmonella enterica serovar Typhimurium were obtained from the human fecal samples. These strains were identified by standard microbiological and biochemical tests. Then, ERIC-PCR and BOX-PCR were carried out for determination of molecular relatedness of Salmonella enterica serovar Typhimurium using specific primers.
Results: The results showed that all 60 Salmonella enterica serovar Typhimurium were separable using ERIC 1, ERIC 2 and BOXAIR. In electrophoresis, 2-11 bands with 20-3200bp for ERIC-PCR and 2-10 bands with 200-1500bp for BOX-PCR were observed. Therefore, 13 different clusters (C1-C13) in ERIC-PCR and 21 different clusters (C1-C21) in BOX-PCR were identified.
Conclusion: The results showed that Salmonella enterica serovar Typhimurium strains were non-homolog. Therefore, ERIC-PCR and BOX-PCR methods are appropriate methods for molecular typing of Salmonella strains and determine the original infection source for the epidemiological survey as well as infection prevention program.

Keywords: Salmonella enterica serovar Typhimurium, BOX-PCR, ERIC-PCR.

Full-Text [PDF 914 kb] (1091 Downloads)

Type of Study: Original article | Subject: Microbiology
Received: 2016/06/10 | Accepted: 2016/12/24 | Published: 2017/11/15

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