Effect of resveratrol the expression of some genes involved epigenetic in breast cancer cell lines (MCF-7, MDA-MB-453)

Ahmadirad , Hadis; Hajizadeh , Mohammad Reza Hajizadeh; Mahmoodi , Mehdi; Mirzaee , Mohamadreza; Shahrbabaki , Fahime Mohammadain; Soltaninejad , Mostafa

Volume 25, Issue 7 (oct 2017) JSSU 2017, 25(7): 526-536 | Back to browse issues page

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Ahmadirad H, Hajizadeh M R H, Mahmoodi M, Mirzaee M, Shahrbabaki F M, Soltaninejad M. Effect of resveratrol the expression of some genes involved epigenetic in breast cancer cell lines (MCF-7, MDA-MB-453). JSSU 2017; 25 (7) :526-536
URL: http://jssu.ssu.ac.ir/article-1-3717-en.html

Effect of resveratrol the expression of some genes involved epigenetic in breast cancer cell lines (MCF-7, MDA-MB-453)

Hadis Ahmadirad

, Mohammad Reza Hajizadeh Hajizadeh ^*

, Mehdi Mahmoodi

, Mohamadreza Mirzaee

, Fahime Mohammadain Shahrbabaki

, Mostafa Soltaninejad

Abstract: (4546 Views)

Background: Breast cancer is one of the most common cancers among women. Incorrect pattern of gene expression involved in epigenetic Including APOBEC3B, DNMT-1 and TET_1 can develop breast cancer.
resveratrol is a natural flavonoids that antioxidant and anti-cancer properties have been reported in other studies. To investigate the mechanism of effect of resveratrol, this study examined effect of resveratrol on the expression of genes referred to the two classes of breast cancer cell lines

Materials and methods: cell lines, including MCF-7 and MDA-MB-453 in separate boxes including the control group and treated groups with two doses of two doses of 25 and 100 micro molar for 24 hours of resveratrol for were cultured. RNA was extracted from the cells and then converted to cDNA. realtime PCR were used for APOBEC3B and DNMT and TET_1expression.

Results: The results showed that resveratrol decrease in gene expression APOBEC3B and DNMT-1 And increased expression of TET_1 in both of cell lines. .

Discussion: due to satisfactory effects of resveratrol has on breast cancer cells, probably these effects through epigenetic mechanisms applied. However, the final decision needs to be further investigation.

Keywords: Resveratrol, Gene expression, Epigenetics, Cell lines, Breast cancer, MCF-7, MDA-MB-453

Full-Text [PDF 1018 kb] (1479 Downloads)

Type of Study: Original article | Subject: Biochemistry
Received: 2016/05/9 | Accepted: 2017/03/11 | Published: 2017/11/13

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