Volume 24, Issue 3 (Jun 2016)                   JSSU 2016, 24(3): 222-231 | Back to browse issues page

XML Persian Abstract Print

Abstract:   (4895 Views)

Introduction: Methamphetamine (MAMP) is a central nervous system stimulant that its consumption has increased among youths. Adolescence is considered as a critical period and the reproductive organs are developing is very sensitive so that the. In this study, the effects of methamphetamine on the development of the testes in immature male rats were evaluated.

Methods: 40 immature male Wistar Rats (35-day-old male rats) were divided into four groups: control and the group receiving methamphetamine: (1, 3, 5 mg/kg). The control group received saline and experimental groups received methamphetamine for 10 days (intraperitoneally) and they were allowed to mature. Then, the rats were dissected. Testicularb tissue was isolated and after weighing, they were prepared to be placed in formalin. The results were analyzed by ANOVA and Duncanchr('39')s test.

Results: Spaces created in the seminiferous tubules showed spermatogenesis disorder. The mean number of cells (spermatogonia, spermatocyte, spermatid, sperm) in the experimental groups decreased significantly than the control group (p<0/001), this reduction was dose dependent. The average weight of the testes to the body weight in the experimental groups showed no significant difference than the control group.

Conclusion: Frequent consumption of methamphetamine, even in low doses through the influence on the pituitary -gonad and the various factors involved in spermatogenesis, can disrupt the sexual maturation, which may cause reduced fertility in males.

Full-Text [PDF 892 kb]   (1519 Downloads)    
Type of Study: Original article | Subject: Biology
Received: 2016/04/14 | Accepted: 2016/06/5 | Published: 2016/09/11

1. Sadock BJ, Sadock VA, Ruiz P. Kaplan and Sadock's Comprehensive Textbook of Psychiatry 10th Edition. Wolters Kluwer Publication.
2. Comer SD, Hart CL, Ward AS, Haney M, Foltin RW, Fischman MW. Effects of repeated oral methamphetamine administration in humans. Psychopharmacology (Berl) 2001; 155(4): 397-404.
3. O'Malley P. Ecstasy for intimacy: Potentially fatal choices for adolescents and young adults: update for the clinical nurse specialist. Clin Nurse Spec 2005; 19(2): 63-4.
4. Kalant H. The pharmacology and toxicology of ecstasy (MDMA) and related drugs. CMAJ 2001; 165(7): 917-28.
5. Jacobs LJ. Reversible dilated cardiomyopathy induced by methamphetamine. Clin Cardiol 1989; 12: 725-27.
6. Kasirsky G. Teratogenic effects of methamphetamine in mice and rabbits. J Am Osteopath Assoc 1971; 70(10): 119-20.
7. Mirjalili1 T, Kalantar SM, Shams Lahijani M, Sheikhha MH, Talebi AR. Congenital effects of methamphetamine on mice fetouses at histological, cellular and chromosomal levels- Iran J Reprod Med 2013; 11(1): 39-46.
8. Inoue H, Nakatome M, Terada M, Mizuno M, Ono R, Iino M, et al. Maternal methamphetamine administration during Pregnancy influences on fetal rat heart development. Life Sci 2004; 74(12): 1529-40.
9. Jarvis S, Elliott DJ, Morgan D, Winston R, Readhead C. Molecular markers for the assessment of postnatal male germ cell development in the mouse. Hum Reprod 2005; 20(1): 108-16.
10. Wang X. The expanding role of mitochondria in apoptosis. Genes Dev 2001; 15(22): 2922-33.
11. Carmen M, Bernal-Manas CM, Morales E, Pastor LM, Pinart E, Bonet S, et al. Proliferation and apoptosis of spermatogonia in postpuberal boar (Sus domesticus) testes with spontaneous unilateral and bilateral abdominal cryptorchidism. Acta Histochem 2005; 107(5): 365-72.
12. Yamamoto Y, Yamamoto K, Hayase T, Abiru H,Shiota K, Mori C. Methamphetamine induces apoptosis in seminiferous tubules in male mice testis. Toxicol Appl Pharmacol 2002; 178(3):155-60.
13. Taghavi MM, Moallem SA, Alavi SH. The Evaluation of Single Dose Effects of Methamphetamine on Proliferation and Apoptosis of Sperm Germ Cells in Mature Rat. J Isfahan Med School 2009; 27(97). [Persian]
14. Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain 1983; 16(2): 109-10.
15. Heidari-Rarani M, Noori A, Ghodousi A. Effects of Methamphetamine on Pituitary Gonadal Axis and Spermatogenesis in Mature Male Rats. Zahedan J Res Med Sci 2014; 16(12): 35-40.
16. Hesami Z,; Khatamsaz S,; Mokhtari M, The Effects of Ecstasy on Pituitary-Gonadal Axis and Spermatogenesis in Mature Male Rats. Dept Physio Faculty Med Kazeroon Azad Univ Kazeroon Iran 2008; 207-18. [Persian]
17. Frith CH, Chang LW, Lattin DL, et al. Toxicology of methylenedioximethamphetamine(MDMA) in the dog and the rat. Fundamental and appl toxico 1987; 9: 110-19.
18. Cobreros BG, Cenrrruzabeitia SL. Ecstasy - induced toxicity in rat live. Liver 2000; 20: 8.
19. Cadet JL, Sheng P, Rothman R, et al: Attenuation of methamphetamine – induced neurotoxicity in copper /zinc supero- xide dismustase transgenic mice. J Neurochem 1994; 62: 380-83.
20. Van Haaster LH, de Jong FH, Docter R, et al. High neonatal triiodothyronin levels reduce the period of sertoli cell proliferation and accelerate tubular lumen formation in the rat testis, and increase serum inhibin levels. Endocrinology 1993; 133: 755-60.
21. Sprague JE, Banks ML, Cook VJ, et al. Hypothalamic- pituitary- Thyroid axis and sympathetic nervous system involvement in the hyperthermia induced by 3, 4 -methylenedioximethamphetamine (MDMA, ecstasy). American Socifor pharmaco experiment therapeutics 2003; 1-28.
22. Malberg JE, Seiden AS. Small changes in ambient tempreture cause large changes in 3,4-methylenedioxymethamphetamine (MDMA) - induced serotonin neurotoxicity and core body tempreture rat . J Neurosci 1998; 18: 5086-94.
23. Nash JF, Meltzer HY, Gudelsky GA. Elevation of serum prolactin and corticosterone concentrations in the rat after the administration of 3,4 methylene dioxy methamphetamine. J Pharmaco experiment therapeutics 1988; 245: 873-79