Volume 24, Issue 3 (Jun 2016)                   JSSU 2016, 24(3): 201-210 | Back to browse issues page

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Abstract:   (5990 Views)

Introduction: Medicinal plants have been a focus of attention to researchers due to their fewer side effects in treating diseases comared to chemical drugs. Some health properties of medicinal plants such as anti-hypercholesterolemia and antioxidant effects of Potentilla reptans have been proven. Therefore, the present study aimed to investigate the antinociceptive and analgesic effect of hydro-alcoholic extract of Potentilla reptans L. (HEP) in the male rat.

Methods: In this experimental study, 36 male rats were divided into 6 groups, including: control group (normal saline), morphine group (1mg/kg), HEP groups (treated with 25, 50 and 100 mg/kg), and naloxone group (200 mg/kg). Writhing, formalin and tail-flick tests were used to evaluate the analgesic effects of the extract. In order to analyze the study data, variance and Tukey test were applied.

Results: The results of the present study demonstrated that in writhing test (P<0.05 and P<0.001 respectively), formalin test (P<0.05, P<0.001) and tail-flick test (P<0.05, P<0.01); the injection of 50, and 100mg/kg dose of the extract produced significant analgesic effects compared with the control group.  50mg/kg dose of Naloxone injection extract has shown a significant analgesic effect in the formalin test (P<0.05). In addition, the effect of the analgesic dose of 100mg/kg of the extract in formalin test was similar to that of morphine.

Conclusions: The findings of the current study revealed that Potentilla reptans L. (HEP) has a significant analgesic effect. Opioid pathway seems to be one of the possible mechanisms of extract effects, which further clinical trials are recommended  in this case.

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Type of Study: Original article | Subject: Pharmacology
Received: 2015/03/15 | Accepted: 2015/10/3 | Published: 2016/06/6

1. Goldman L, Bennett JC. Cecil textbook of medicine, 21 th edit. WB SaundersCo 2000; 103-04.
2. Weiner RS. Pain management: A Practical Guide for Clinicians. 6rd ed. Florida: CRC Press Inc 2001, 3-9.
3. Wal PD, Melozoc R. Textbook of pain. Vol.1. 2nd ed. churchill livengstone 2006, 110-15.
4. Saller S, Iten F, Reichling J. Dyspepsia pain and phytotherapy- a review of traditional and modern herbal drugs. For sch Komplementarmed Klass Naturheilkd 2001; 8(5): 263-73.
5. Gurbuz I, Ozkan AM, Yesilada E, Kutsal O. Anti-ulcerogenic activity of some plants used in folk medicine of Pinarbasi. J Ethnopharmacol 2005; 101(1): 313-18.
6. Zargari A. Medicinal Plants, Tehran: Tehran University Publications; 1990; 4: 42-5.
7. De Natale A, Pollio A. Plant species in the folk medicine of Montecorvino Rovella (inland Campania, Italy). J Ethnopharmacol 2007; 109(2): 295-303.
8. Gürbüz I, Özkan AM, Yesilada E, Kutsal O. Anti-ulcerogenic activity of some plants used in folk medicine of Pinarbasi (Kayseri, Turkey). J Ethnopharmacol 2005; 101(1): 313-18.
9. ´Swie˙zewska E, Chojnacki T. The occurrence of unique, long-chain polyprenols in the leaves of Potentilla species. Acta Biochimica Polonica 1989; 36: 143-58.
10. Iva nova D, Ger ova D, Chervenkov T, Yank ova T. Polyphenols and antioxidant capacity of Bulgarian medicinal plants. J Ethnopharmacol 2005; 96(1): 145-50.
11. Avci G, Kupeli E, Eryavuz A, Yesilada E, Kucukkurt I. Antihypercholesterolaemic and antioxidant activity assessment of some plants used as remedy in Turkish folk medicine. J Ethnopharmacol 2006; 107(3): 418-23.
12. Mahmoodi M, Mohammadi S, Zarei M. Antinociceptive Effect of Hydroalcoholic Leaf Extract of Tribulus Terrestris L. in Male Rat. J Babol Univ Med ci 2013; 5(6): 36-43. [persian]
13. Mohammadi S, Zarei M, Mahmoodi M, Zarei MM, Nematian MA. In Vivo Antinociceptive Effects of Persian Shallot (Allium hirtifolium) in Male Rat. Avicenna J Neuro Psych Physio 2015; 2(1): 27-37. [persian]
14. Asgari Nematian M, Mohammadi S. The Evaluation of the Analgesic Effects and Acute Toxicity of Methanol Extract of Pimpinella anisum. L in Male Wistar Rats. J Babol Univ Med Sci 2015; 17(5): 59-65 . [persian]
15. Zarei M, Mohammadi S, Abolhassani N, Asgari Nematian M. The Antinociceptive Effects of Hydroalcoholic Extract of Bryonia dioica in Male Rats. Avicenna J Neuro Psych Physio 2015; 2(1): 18-25. [Persian]
16. Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain 1983; 16(2): 109-10.
17. Collier HOJ, Dinneen LC, Johnson CA, Schneider C. The abdominal constriction response and its suppression by analgesic drugs in the mouse. Br J Pharmacol 1968; 32(2): 295-310.
18. D’Amour FE, Smith DL. A method of determining loss of pain sensation. J Pharmacol Exp Ther 1941; 27: 74-7.
19. Dubuisson D, Dennis SG. The formalin test: a quantitative study of the analgesic effect of morphine, meperidine, and brain stem stimulation in rats and cats. Pain 1978; 4: 161-74.
20. Assefa B, Glatzel G, Buchmann C. Ethnomedicinal uses of Hagenia abyssinica (Bruce) J.F. Gmel. among rural commu Ethiopia. J Ethnobio Ethnomedicine 2010; 6(1): 1-10.
21. Negus SS, Vanderah TW, Brandt MR, Bilsky EJ, Becerra L, Borsook D. Preclinical assessment of candidate analgesic drugs: recent advances and future challenges. J Pharmacol Exp Ther 2006; 319(2): 507-14.
22. Gene RM, Segura L, Adzet T, Marin E, Inglesias J. Heterotheca inuloides: anti-inflammatory and analgesic effects. J Ethnopharmacol 1998; 60(2): 57-62.
23. Koster R, Anderson M, De Beer EJ. Acetic acid for analgesic screening. Federation Proceedings 1959; 18(1): 412.
24. Jensen TS, Yaksh TL. Comparison of antinociceptive action of morphine in the periaqueductal gray, medial and paramedial in rat. Brain Res 1986; 363(1): 99-113.
25. Rice AS, Cimino-Brown D, Eisenach JC, Kontinen VK, Lacroix-Fralish ML, Machin I, et al. Animal models and the prediction of efficacy in clinical trials of analgesic drugs: A critical appraisal and call for uniform reporting standards. Pain 2008; 139(2): 243-47.
26. Tjolsen A, Berge OG, Hunskaar S, Rosland JH, Hole K. The formalin test: an evaluation of the method. Pain 1992; 51(1): 5-17.
27. Shibata M, Ohkubo T, Takahashi H, Inoki R. Modified formalin test: characteristic biphasic pain response. Pain 1989; 38(3): 347-52.
28. Verma PR, Joharapurkar AA, Chatpalliwar VA, Asnani AJ. Antinociceptive activity of alcoholic extract of Hemidesmus indicus R.Br. in mice. J Ethnopharmacol 2005; 102(2): 298-301.
29. Vaccarino AL, Tasker RAR, Melzack R. Analgesia produce by normal doses of opioid antagonists alone and in combination with morphin. Pain 989; 36(1): 103-09.
30. Borras MC, Becerra L, Ploghaus A, Gostic JM, Dasilva A, Gonzalez RG, et al. Gonzalez RG, Borsook D. FMRI measurement of cns responses to naloxone infusion and subsequent mild noxious thermal stimuli in healthy volunteers. JN Physiol 2004; 91(6): 2723-33.
31. Bate-Smith EC. Chromatography and taxonomy in the Rosaceae with the special reference to Potentilla and Prunus. Botanical J Linnean Soc 961; 58(370): 39-54.
32. Tomczyk M, Leszczy ´nska K, Tomczykowa M, Jakoniuk P. Screening of antimicrobial activity of aqueous extracts of the selected Potentilla L. species. Planta Med 2007; 73(09): P_100.
33. Krzaczek T. Phenolic acids in some tannin drugs from the Rosaceae family. Farm Pol 1984; 40(8): 475-77.
34. Di Carlo G, Mascolo N, Izzo AA, Capasso F. Flavonoids ,old and new aspects of a class of natural therapeutic drugs. Life Sci 1999; 65(4): 337-53.
35. Özek M, Üresin Y, Güngör M. Comparison of the effects of specific and nonspecific inhibition of nitric oxide synthase on morphine analgesia, tolerance and dependence in mice. Life Sci 2003; 72(17): 1943-51.
36. Di Carlo G, Mascolo N, Izzo AA, Capasso F. Flavonoids ,old and new aspects of a class of natural therapeutic drugs. Life Sci 1999; 65(4): 337-53.
37. Simoes CMO, Schenkel EP, Bauer L, Langeloh A. Pharmacological investigations on Achyrocline satureioides (Lam.) DC, Compositae. J Ethnopharmacol 1988; 22(3): 281-93.
38. Alcaraz MJ, Hoult JRS. Action of flavonoids and the novel anti-inflammatory flavone, Hypolaetin-8-Glucoside, on prostaglandin biosynthesis and inactivation. Bio Pharmacol 1985; 34(14): 2477-82.
39. Starec M, Waitzova D, Elis J. Evaluation of the analgesic effect of RG-tannin using the “hot plate” and “tail flick”method in mice. Cesk Farm 1988; 37: 319-21.