Volume 23, Number 4 (Jun-Jul 2015)                   JSSU 2015, 23(4): 2109-2117 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Kohestanian K, Baharara J, Ramazani T, Mosavi M. Antiangiogenic Effects of Eugenol in Chorioallantoic Membrane of Chick Embryo. JSSU. 2015; 23 (4) :2109-2117
URL: http://jssu.ssu.ac.ir/article-1-3001-en.html

Abstract:   (2308 Views)
Introduction: Eugenol is a substance obtained from clove that has some therapeutic properties. Angiogenesis involves the formation of new blood vessels from the primary vessels. Since today natural products are taken into consideration in regard with anti-angiogenic therapies, this study intended to investigate anti-angiogenic effects of eugenol on chorioallantoic membrane (CAM) of chick embryo. Methods: In this experimental study, 70 Ross fertilized eggs were randomly divided into: control, sham and five experimental groups. On the 8th day of incubation, experimental groups were treated with 0.5 -5 mM of eugenol. On the 12th day, all cases were photographed and the numbers and lengths of vessels around the sponges were measured via applying image J software. Furthermore, the hemoglobin content was measured using Drabkin test, and then the embryo weight was recorded. The study data were analyzed statistically via SPSS software utilizing ANOVA test at significance level of p<0.05. Results: No significant difference was observed in regard with average number and length of vessels in sham and control groups. It is worth mentioning that the average number and length of vessels in experimental groups of 3, 4 and 5 revealed a significant decrease compared to the control group (p<0/001). The hemoglobin content of experimental groups of 2، 3, 4 and 5 demonstrated a significant decrease (p<0/001). Moreover, the weight of the embryos in 3, 4 and 5experimental groups showed a significant decrease compared to the controls (p<0.001). Conclusion: As the study findings revealed, Eugenol was reported to have inhibitory effect on angiogenesis in the chick embryo, therefore, it may be regarded as a good candidate for studies concerned with cancer treatment and pathological conditions associated with angiogenesis.
Full-Text [PDF 673 kb]   (854 Downloads)    
Type of Study: Original article | Subject: Biology
Received: 2014/11/21 | Accepted: 2015/03/9 | Published: 2015/07/7

Add your comments about this article : Your username or email:
Write the security code in the box

Send email to the article author

© 2015 All Rights Reserved | SSU_Journals

Designed & Developed by : Yektaweb