Volume 19, Issue 5 (Nov-Dec 2011)
JSSU 2011, 19(5): 568-77 |
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Mohseni Kouchesfahani, Nabiuni, Delaviz, Bahrebar, Gheibi, Eslami. Differentiation Induction of the Mesenchymal Stem Cells Derived from Bone Marrow to Lens Fiber-Like Cells . JSSU 2011; 19 (5) :568-77
URL: http://jssu.ssu.ac.ir/article-1-1732-en.html
URL: http://jssu.ssu.ac.ir/article-1-1732-en.html
Abstract: (10335 Views)
Introduction: In this study the effect of vitreous humor on the mesenchymal stem cells (MSCs) derived from bone marrow to lens fiber-like cells was investigated.
Methods: In this experimental study bone marrow cells were collected by flushing femurs and tibias in NMRI mice. Immunocytochemistry by Oct4 antibody was used to confirm that the cells are stem cells. The mesenchymal character of these cells was proven by their adherence and by such flocytometery markers as CD44, CD31. In experimental groups, MSCs were cultured with DMEM and bovine vitreous body for induction. The supplemented medium was changed every two days. The expression of α –crystalline, as the marker of lens differentiation, was detected by immunocytochemistry.
Results: During the primary culture, the cell population was heterogeneous where varying morphologies such as flat, spindle-shaped, and polygonal were observed. In the subsequent passages, the number of the spindle-shaped cells appeared to increase, so that in passage 3 the majority of the cells seemed morphologically to be spindle-shaped.
Immunocytochemistery study confirmed the presence of stem cells using Oct4 antibody. The flowcytometric analysis of surface markers revealed that mesenchymal bone marrow stem cells express CD44 and CD31 to a low level. Morphological studies showed that most cells in experimental groups were locally longer and more aligned in parallel compared to control group cells. α –crystalline expression proved the formation of lens fiber-like cells.
Conclusion: According to the findings of this study, it can be concluded that MSCs derived from mouse bone marrow differentiate into lens fiber like cells by treating them with vitreous humor.
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