Preventive effect of injections of vitamin D on the levels of Klotho in the brain tissue of female lewis rats with experimental autoimmune ensephalomyelitis

fatemi, seyedeh fatemeh; fallah mohammadi, zia; Talebi, vahid; Hosseini, Sayed mojtaba

doi:10.18502/ssu.v26i8.238

Volume 26, Issue 8 (Nov 2019) JSSU 2019, 26(8): 662-670 | Back to browse issues page

‎ 10.18502/ssu.v26i8.238

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fatemi S F, fallah mohammadi Z, Talebi V, Hosseini S M. Preventive effect of injections of vitamin D on the levels of Klotho in the brain tissue of female lewis rats with experimental autoimmune ensephalomyelitis. JSSU 2019; 26 (8) :662-670
URL: http://jssu.ssu.ac.ir/article-1-4156-en.html

Preventive effect of injections of vitamin D on the levels of Klotho in the brain tissue of female lewis rats with experimental autoimmune ensephalomyelitis

Seyedeh fatemeh Fatemi

, Zia Fallah mohammadi ^*

, Vahid Talebi

, Sayed mojtaba Hosseini

Abstract: (3435 Views)

Introdution: The aim of this study was to investigate the effect of vitamin D supplementation on Klotho levels of the brain tissue.
Methods: In this laboratory experimental study, 25 female Lewis rats, after 2 weeks adaptation to lab environmental, were randomly divided into 5 groups: 1-control; 2- control+ EAE; 3-sham; 4vitamin D; 5-vitaminD + EAE.
Results: The data were analyzed using one way ANOVA, and to examine the weight changes Mann-Whitney test was used to the research groups. In order to induce EAE, 0.4 ml of solution, a combination of guinea Hindi pig spinal cord and adjuvant ratio was injected to each animal. Klotho levels were measured by ELISA.
Finding: There was significantly different in Klotho levels of brain tissue of control+ EAE compared to control group (P=0/001). There was significant difference in Klotho levels of the brain tissue in EAE +vitamin D group compared to control group (P=0/001). In addition, the weight of vitamin D+EAE group was more than control+EAE group (P=0/006).
Conclusion: Pre-treatment with vitamin D supplementation increases the Klotho levels of brain tissue and may have protective role against EAE.

Keywords: Experimental autoimmune encephalomyelitis, Vitamin D, Klotho.

Full-Text [PDF 972 kb] (1002 Downloads)

Type of Study: Original article | Subject: Neurology
Received: 2017/04/10 | Accepted: 2018/06/23 | Published: 2018/12/25

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