Response of Estrogen-related Receptor Alpha (ERRα) to Endurance Training and its Participation in Endurance Training-induced Adaptations in Lipid Metabolism in Skeletal Muscle of Male Wistar rats

Aminizadeh , Soheil; Habibi , Abdolhamid; Marefati , Hamid; Shakerian , Saeed

Volume 25, Issue 5 (Jul-Aug 2017) JSSU 2017, 25(5): 414-425 | Back to browse issues page

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Aminizadeh S, Habibi A, Marefati H, Shakerian S. Response of Estrogen-related Receptor Alpha (ERRα) to Endurance Training and its Participation in Endurance Training-induced Adaptations in Lipid Metabolism in Skeletal Muscle of Male Wistar rats . JSSU 2017; 25 (5) :414-425
URL: http://jssu.ssu.ac.ir/article-1-4083-en.html

Response of Estrogen-related Receptor Alpha (ERRα) to Endurance Training and its Participation in Endurance Training-induced Adaptations in Lipid Metabolism in Skeletal Muscle of Male Wistar rats

Soheil Aminizadeh ^*

, Abdolhamid Habibi

, Hamid Marefati

, Saeed Shakerian

Abstract: (5053 Views)

Introduction: The aim of the present study was to investigate the expression of estrogen-related receptor alpha (ERRα) - an important factor in cellular energy homeostasis regulation - in skeletal muscle of male Wistar rats after four weeks of endurance training and to determine its role with fat metabolism indexes.
Methods: In this experimental study, 30 male Wistar rats (eight weeks-old) were randomly divided into four groups: control (n=7), control+XCT790 (n=8), endurance training (n=8), and endurance training+XCT790 (n=7). ERRα was inhibited by intraperitoneal injection of XCT790 (0.48 mg/kg.day) on daily bases. Four weeks of endurance training (five times per week) started at 15 m/min for 20 min and reached to 27 m/min for 50 min was performed by the animals from trained groups. Expression of ERRα, Medium-chain acyl-CoA dehydrogenase (MCAD) and Carnitine Palmitoyl Transferase 1 (CPT-1β) mRNA was measured by Real-Time PCR and quantified by method. One-way analyses of variance were used for cross-group comparison.
Results: The expression of ERRα (P˂0.037), MCAD (P˂0.001), and CPT1β (P˂0.001) mRNA in endurance training group was significantly higher than the control group. The expression of MCAD (P˂0.001) and CPT1β mRNA (P˂0.001) in the endurance training+XCT790 group was significantly lower compared to those values of the endurance training group.
Conclusion: In sum, expression of ERRα is a trainable factor and its changes are parallel with the increase in expression of lipid metabolism indexes; so, it could have a direct role in endurance training-induced adaptation in fat metabolism.

Keywords: ERRα, Lipid metabolism, Endurance training, Oxidative phosphorylation

Full-Text [PDF 991 kb] (1218 Downloads)

Type of Study: Original article | Subject: Exercise Physiology
Received: 2017/01/22 | Accepted: 2017/05/20 | Published: 2017/10/14

References

1. Smekal G, von Duvillard SP, Pokan R, Tschan H, Baron R, Hofmann P, et al. Effect of endurance training on muscle fat metabolism during prolonged exercise: agreements and disagreements. Nutrition 2003; 19(10): 891-900.

2. Horowitz JF, Klein S. Lipid metabolism during endurance exercise. Am J Clin Nutr 2000; 72(2): 558S-63S.

3. Talanian JL, Holloway GP, Snook LA, Heigenhauser GJ, Bonen A, Spriet LL. Exercise training increases sarcolemmal and mitochondrial fatty acid transport proteins in human skeletal muscle. Am J Physiol Endocrinol Metab 2010; 299(2): E180-8.

4. Chen X, Zhang F, Gong Q, Cui A, Zhuo S, Hu Z, et al. Hepatic ATF6 Increases Fatty Acid Oxidation to Attenuate Hepatic Steatosis in Mice Through Peroxisome Proliferator-Activated Receptor alpha. Diabetes 2016; 65(7): 1904-15.

5. Pedraza-Chaverri J, Sanchez-Lozada LG, Osorio-Alonso H, Tapia E, Scholze A. New Pathogenic Concepts and Therapeutic Approaches to Oxidative Stress in Chronic Kidney Disease. Oxid Med Cell Longev 2016; 2016: 6043601.

6. Suwa M, Nakano H, Radak Z, Kumagai S. Endurance exercise increases the SIRT1 and peroxisome proliferator-activated receptor gamma coactivator-1alpha protein expressions in rat skeletal muscle. Metabolism 2008; 57(7): 986-98.

7. Miljkovic I, Yerges LM, Li H, Gordon CL, Goodpaster BH, Kuller LH, et al. Association of the

8. CPT1B gene with skeletal muscle fat infiltration in Afro-Caribbean men. Obesity (Silver Spring) 2009; 17(7): 1396-401.

9. Tunstall RJ, Mehan KA, Wadley GD, Collier GR, Bonen A, Hargreaves M, et al. Exercise training increases lipid metabolism gene expression in human skeletal muscle. Am J Physiol Endocrinol Metab 2002; 283(1): E66-72.

10. Murray J, Auwerx J, Huss JM. Impaired myogenesis in estrogen-related receptor gamma (ERRgamma)-deficient skeletal myocytes due to oxidative stress. FASEB J 2013; 27(1): 135-50.

11. Giguere V. Transcriptional control of energy homeostasis by the estrogen-related receptors. Endocr Rev 2008; 29(6): 677-96.

12. Tremblay AM, Giguere V. The NR3B subgroup: an ovERRview. Nucl Recept Signal 2007; 5: e009.

13. Huss JM, Torra IP, Staels B, Giguere V, Kelly DP. Estrogen-related receptor alpha directs peroxisome proliferator-activated receptor alpha signaling in the transcriptional control of energy metabolism in cardiac and skeletal muscle. Mol Cell Biol 2004; 24(20): 9079-91.

14. Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, et al. Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria. Cell 2003; 115(5): 629-40.

15. Sladek R, Bader JA, Giguere V. The orphan nuclear receptor estrogen-related receptor alpha is a transcriptional regulator of the human medium-chain acyl coenzyme A dehydrogenase gene. Mol Cell Biol 1997; 17(9): 5400-9.

16. Cartoni R, Leger B, Hock MB, Praz M, Crettenand A, Pich S, et al. Mitofusins 1/2 and ERRalpha expression are increased in human skeletal muscle after physical exercise. J Physiol 2005; 567(1): 349-58.

17. Wallace MA, Lamon S, Russell AP. The regulation and function of the striated muscle activator of rho signaling (STARS) protein. Front Physiol 2012; 3: 469.

18. Hu JZ, Long H, Wu TD, Zhou Y, Lu HB. The effect of estrogen-related receptor alpha on the regulation of angiogenesis after spinal cord injury. Neuroscience 2015; 80: 570-80.

19. Teyssier C, Bianco S, Lanvin O, Vanacker JM. The orphan receptor ERRalpha interferes with steroid signaling. Nucleic Acids Res 2008; 36(16): 5350-61.

20. Eskiocak B, Ali A, White MA. The estrogen-related receptor alpha inverse agonist XCT 790 is a nanomolar mitochondrial uncoupler. Biochemistry 2014; 53(29): 4839-46.

21. Mansouri M, Nikooie R, Keshtkar A, Larijani B, Omidfar K. Effect of endurance training on retinol-binding protein 4 gene expression and its protein level in adipose tissue and the liver in diabetic rats induced by a high-fat diet and streptozotocin. J Diabetes Investig 2014; 5(5): 484-91. [persion]

22. Lanvin O, Bianco S, Kersual N, Chalbos D, Vanacker JM. Potentiation of ICI182,780 (Fulvestrant)-induced estrogen receptor-alpha degradation by the estrogen receptor-related receptor-alpha inverse agonist XCT790. J Biol Chem 2007; 282(39): 28328-34.

23. Bianco S, Lanvin O, Tribollet V, Macari C, North S, Vanacker JM. Modulating estrogen receptor-related receptor-alpha activity inhibits cell proliferation. J Biol Chem 2009; 284(35): 23286-92.

24. Egan B, Zierath JR. Exercise metabolism and the molecular regulation of skeletal muscle adaptation. Cell Metab 2013; 17(2): 162-84.

25. Deblois G, Giguere V. Functional and physiological genomics of estrogen-related receptors (ERRs) in health and disease. Biochim Biophys Acta 2011; 1812(8): 1032-40.

26. Hoydal MA, Wisloff U, Kemi OJ, Ellingsen O. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. Eur J Cardiovasc Prev Rehabil. 2007; 14(6): 753-60.

27. Berthon PM, Howlett RA, Heigenhauser GJ, Spriet LL. Human skeletal muscle carnitine palmitoyltransferase I activity determined in isolated intact mitochondria. J Appl Physiol 1998; 85(1): 148-53.

28. Starritt EC, Howlett RA, Heigenhauser GJ, Spriet LL. Sensitivity of CPT I to malonyl-CoA in trained and untrained human skeletal muscle. Am J Physiol Endocrinol Metab 2000; 278(3): E462-8.

29. Jong-Yeon K, Hickner RC, Dohm GL, Houmard JA. Long- and medium-chain fatty acid oxidation is increased in exercise-trained human skeletal muscle. Metabolism 2002; 51(4): 460-4.

30. Wende AR, Huss JM, Schaeffer PJ, Giguere V, Kelly DP. PGC-1alpha coactivates PDK4 gene expression via the orphan nuclear receptor ERRalpha: a mechanism for transcriptional control of muscle glucose metabolism. Mol Cell Biol 2005; 25(24): 10684-94.