Volume 24, Issue 10 (Jan 2017)                   JSSU 2017, 24(10): 828-839 | Back to browse issues page

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Khademi Y, Azarbayejani M, Hosseini S. The Effects of Combination of High Intensity Intermittent Exercise and Supplement Flaxseed Oil 1 ICAM- Plasma Concentration in Male Rats. JSSU. 2017; 24 (10) :828-839
URL: http://jssu.ssu.ac.ir/article-1-3890-en.html
Abstract:   (5699 Views)

Introduction: Prevalence of cardiovascular diseases is rooted in inflammation and inflammation is along with the increase of ICAM-1. The aim of the present study was to review the effect of combination of high interval training and flaxseed oil supplementation on plasma concentration of ICAM-1 of rats.

Methods: In this experimental study, 30 rats were selected and divided into six groups of five male rats, including (1) control, (2) training, (3) 10 mg/kg Flaxseed oil, (4) 30 mg/kg Flaxseed oil, (5) training with 10 mg/kg Flaxseed oil and (6) training with 30 mg/kg Flaxseed oil. Groups of 2, 5 and 6 were trained with high intensity interval training for 10 weeks and five sessions per week, also groups 3 to 6 received flaxseed oil for 10 weeks. For statistical analysis of data two way ANOVA test was used (p≤0.05).

Results: 10 weeks  treating high intensity interval training  for 10 weeks had significant effect on the increase of the plasma concentration of ICAM-1 of male rats (p=0.001). Nevertheless, Taking 10 weeks 10 and 30 mg/kg flaxseed oil supplementation for 10 weeks has significant effect on reducing  the plasma concentration of ICAM-1 of rats (p=0.001). Training and taking flaxseed oil supplementation have interactional effect on reduingc the plasma concentration of ICAM-1 of male rats (p=0.001).

Conclusion: Combination of high intensity interval training and flaxseed oil supplementation has interactional effects on the improvement of plasma concentration of ICAM-1 in male rats.

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Type of Study: Original article | Subject: Exercise Physiology
Received: 2016/09/19 | Accepted: 2016/11/26 | Published: 2017/02/4

1. Hopper CA, Gruber MB, Munoz KD, MacConnie SE, Pfingston YM, Nguyen K. Relationship of blood cholesterol to body composition, physical fitness, and dietary intake measures in third-grade children and their parents. Res Q Exerc Sport 2001; 72 (2): 182- 8.
2. Blake GJ, Ridker PM. Inflammatory bio-markers and cardiovascular risk prediction. J Intern Med 2002; 252 (4): 283- 94.
3. Afzalpour M, Gharakhanlou R, Gaeini A, Mohebbi H, Hedayati M, Khazaei M. The effects of aerobic exercises on the serum oxidized LDL and total antioxidant capacity in non-active men. CVD prevention and control 2008; 3(2):77-82.
4. Blake GJ, Ridker PM . Novel clinical markers of vascular wall inflammation. Circulation research 2001; 89(9):763-71.
5. Witkowska AM. Soluble ICAM-1: a marker of vascular inflammation and lifestyle. Cytokine 2005; 31 (2): 127- 34.
6. Geffken DF, Cushman M, Burke GL, Polak JF, Sakkinen PA, Tracy RP. Association between physical activity and markers of inflammation in a healthy elderly population. Am J Epidemiol 2001; 153(3): 242- 50.
7. Ding YH, Young CN, Luan X, Li J, Rafols JA, Clark JC, et al. Exercise preconditioning ameliorates inflammatory injury in ischemic rats during reperfusion. Acta Neuropathol 2005; 109(3): 237-246.
8. Wang RY, Yang YR, Yu SM. Protective effects of treadmill training on infarction in rats. Brain Res 2001; 922 (1): 140- 3.
9. Adamopoulos S, Parissis J, Kroupis C, Georgiadis M, Karatzas D, Karavolias G, et al. Physical training reduces peripheral markers of inflammation in patients with chronic heart failure. Eur Heart J 2001; 22 (9): 791- 7.
10. Rehman J, Mills PJ, Carter SM, Chou J, Thomas J, Maisel AS. Dynamic exercise leads to an increase in circulating ICAM-1: further evidence for adrenergic modulation of cell adhesion. Brain Behav Immun 1997; 11 (4): 343- 51.
11. Akimoto T, Furudate M, Saitoh M, Sugiura K, Waku T, Akama T, et al. Increased plasma concentrations of intercellular adhesion molecule-1 after strenuous exercise associated with muscle damage. Eur J Appl Physiol 2002; 86 (3): 185- 90.
12. Lupattelli G, Lombardini R, Schillaci G, Ciuffetti G, Marchesi S, Siepi D, et al. Flow-mediated vasoactivity and circulating adhesion molecules in hypertriglyceridemia: association with small, dense LDL cholesterol particles. Am Heart J 2000; 140 (3): 521- 6.
13. Hammett CJ, Prapavessis H, Baldi JC, Varo N, Schoenbeck U, Ameratunga R, et al. Effects of exercise training on 5 inflammatory markers associated with cardiovascular risk. Am Heart J 2006; 151 (2): 367- 76.
14. Nassis GP, Papantakou K, Skenderi K, Triandafillopoulou M, Kavouras SA, Yannakoulia M, et al. Aerobic exercise training improves insulin sensitivity without changes in body weight, body fat, adiponectin, and inflammatory markers in overweight and obese girls. Metabolism 2005; 54 (11): 1472- 9.
15. Sabatier MJ, Schwark EH, Lewis R, Sloan G, Cannon J, McCully K. Femoral artery remodeling after aerobic exercise training without weight loss in women. Dyn Med 2008;7: 13.
16. Ziccardi P, Nappo F, Giugliano G, Esposito K, Marfella R, Cioffi M, et al. Reduction of inflammatory cytokine concentrations and improvement of endothelial functions in obese women after weight loss over one year. Circulation 2002; 105 (7): 804- 9.
17. Ding YH, Young CN, Luan X, Li J, Rafols JA, Clark JC, et al. Exercise preconditioning ameliorates inflammatory injury in ischemic rats during reperfusion. Acta Neuropathol 2005; 109 (3): 237- 46.
18. Abednatanzi A, Choopani Z. The effect of six weeks of high intensity interval training (HIIT) on plasmatic levels of cellular adhesion molecules (ICAM-1) and lipid profile in young overweight women. Int Res J Appl Basic Sci 2014; 8 (11): 2082- 2088.
19. Blaiche J, Burette A, De Vos M, Louis E, Huybrechts M, Deltenre M. Observational survey of NSAID-related upper gastro-intestinal adverse events in Belgium. Acta Gastroenterol Belg 2002; 65(2):65-73.
20. Tofighi A, Ghafari Gh. Effect of aerobic training with omega-3 consumption on soloble adhesion molecules in the obese women. Med J Tabriz Univ Med Sci 2014; 36(1): 20-27.
21. Jalali F, Hajian K, Baradaran M, Moghaddamnia AA. Effect of Linseed (Seed of Flax) on blood lipid levels. Pajoohandeh Journal 2008;13(2): 107-13.
22. Kaithwas G, Mukherjee A, Chaurasia AK, Majumdar D. Anti-inflammatory, analgesic and antipyretic activities of Linum usitatissimum L (flaxseed/linseed) fixed oil. Indian J Exp Biol 2011;49(12):932-8.
23. Deng Y, Zhang CH, Zhang HN. Effects of chaihu shugan powder on the behavior and expressions of BDNF and TrkB in the hippocampus, amygdala, and the frontal lobe in rat model of depression. Zhongguo Zhong Xi Yi Jie He Za Zhi 2011; 31 (10): 1373- 8.
24. Chytilova M, Mudronova D, Nemcova R, Gancarcikova S, Buleca V, Koscova J, et al. Anti-inflammatory and immunoregulatory effects of flax-seed oil and Lactobacillus plantarum - Biocenol LP96 in gnotobiotic pigs challenged with enterotoxigenic Escherichia coli. Res Vet Sci 2013; 95 (1): 103- 9.
25. Chytilova M, Nemcova R, Gancarcikova S, Mudronova D, Tkacikova L. Flax-seed oil and Lactobacillus plantarum supplementation modulate TLR and NF-kappaB gene expression in enterotoxigenic Escherichia coli challenged gnotobiotic pigs. Acta Vet Hung 2014; 62 (4): 463- 72.
26. Green AG. Genetic control of polyunsaturated fatty acid biosynthesis in flax (Linum usitatissimum) seed oil. Theor Appl Genet 1986; 72 (5): 654- 61.
27. Tanna IR, Aghera HB, Ashok BK, Chandola HM. Protective role of Ashwagandharishta and flax seed oil against maximal electroshock induced seizures in albino rats. Ayu 2012; 33 (1): 114- 8.
28. Tuluce Y, Ozkol H, and Koyuncu I. Photoprotective effect of flax seed oil (Linum usitatissimum L.) against ultraviolet C-induced apoptosis and oxidative stress in rats. Toxicol Ind Health 2012; 28 (2): 99- 107.
29. Williams D, Verghese M, Walker LT, Boateng J, Shackelford L, Chawan CB. Flax seed oil and flax seed meal reduce the formation of aberrant crypt foci (ACF) in azoxymethane-induced colon cancer in Fisher 344 male rats. Food Chem Toxicol 2007; 45 (1): 153- 9.
30. Sanadgol N, Mostafaie A, Mansouri K, Bahrami G. Effect of palmitic acid and linoleic acid on expression of ICAM-1 and VCAM-1 in human bone marrow endothelial cells (HBMECs). Arch Med Sci 2012; 8 (2): 192-8.
31. Papageorgiou N, Tousoulis D, Psaltopoulou T, Giolis A, Antoniades C, Tsiamis E, et al. Divergent anti-inflammatory effects of different oil acute consumption on healthy individuals. Eur J Clin Nutr 2011; 65 (4): 514- 9.
32. Han H, Yan P, Chen L, Luo C, Gao H, Deng Q, et al. Flaxseed oil containing alpha -linolenic acid ester of plant sterol improved atherosclerosis in apo E deficient mice. Oxid Med Cell Longev 2015; 2015: 958217.
33. El-Sharaky N, Eweda B. Bromobenzene-induced hepatotoxicity in male rats: the protective effect of flaxseed. Toxicological Environmental Chem 2012; 37- 41.
34. Ghanbari-Niaki A, Rahmati-Ahmadabad S. Effects of a fixed-intensity of endurance training and pistacia atlantica supplementation on ATP-binding cassette G4 expression. Chin Med 2013; 8 (1): 23.
35. Shafiee A, kordi M, Gaeini A, Soleimani M, Nekouei A, Hadidi V. The effect of eight week of high intensity interval training on expression of mir-210 and ephrinA3 mRNA in soleus muscle healthy male rats. Arak Univ Med Sci J 2014; 17 (3): 26- 34.
36. Petridou A, Chatzinikolaou A, Fatouros I, Mastorakos G, Mitrakou A, Chandrinou H, et al. Resistance exercise does not affect the serum concentrations of cell adhesion molecules. Br J Sports Med 2007; 41 (2): 76- 9.
37. Ciuffetti G, Lombardini R, Pasqualini L, Vaudo G, Lupattelli G. Circulating leucocyte adhesion molecules in chronic venous insufficiency. Vasa 1999; 28 (3): 156- 9.
38. Mizia-Stec K, Zahorska-Markiewicz B, Mandecki T, Janowska J, Szulc A, Jastrzebska-Maj E. Serum levels of selected adhesion molecules in patients with coronary artery disease. Int J Cardiol 2002; 83 (2): 143- 50.
39. Wang JS, Chen YW, Chow SE, Ou HC, Sheu WH. Exercise paradoxically modulates oxidized low density lipoprotein-induced adhesion molecules expression and trans-endothelial migration of monocyte in men. Thromb Haemost 2005; 94 (4): 846- 52.
40. Kahkha H, Nasrabadi R, Nuraeinjar M. Effect of twelve weeks aerobic training on selected molecules 1, TNF- α, CRP, ICAM-1, VCAM-1 type 2 diabetes in middle-aged women. J Sci Res Development 2015; 2 (1): 154- 157.
41. Jalaly L, Sharifi G, Faramarzi M, Nematollahi A, Rafieian-kopaei M, Amiri M, et al. Comparison of the effects of crataegus oxyacantha extract, aerobic exercise and their combination on the serum levels of ICAM-1 and E-Selectin in patients with stable angina pectoris. Daru J 2015; 19: 23- 30.
42. Nikbakht H, Gaeini A, Mohammadzadeh Salamat K. The effect of endurance training on serum adhesion molecules in overweight men. Kurdistan Med Sci Uni J 2016; 21 (3): 79- 88.
43. Farsi S, Azarvayjani MA, Hosseini SA, Piree M. Response of serum levels of ICAM-1, VCAM- 1 and CRP to High and moderate endurance trainings in sprague dawley male diabetic rats. Armaghane danesh J 2016; 21 (8): 757- 786.
44. Nielsen HG, Lyberg T. Long-distance running modulates the expression of leucocyte and endothelial adhesion molecules. Scand J Immunol 2004; 60 (4): 356- 62.
45. Mogharnasi M, Gaeini AA, Sheikholeslami-Vatani D. Changes in pre-inflammatory cytokines and markers of vascular inflammation after regular endurance training. ZJRMS 2008; 10 (2): 125- 135.
46. Scheede-Bergdahl C, Benee Olsen D, Reving D, Boushel R, Dela F. Cardiovascular disease markers in type 2 diabetes: the effects of a moderate home based exercise training program. Diab Vasc Dis Res 2009; 6 (4): 291- 296.
47. Tonjes A, Schola M, Fasshauer M, Kratzsch J, Rassoul F, Stumvoll M, et al. Beneficial effects of a 4-week exercise program on plasma concentrations of adhesion molecules. diabetes care 2007; 30: 1-2.
48. Mirfatahi M, Tabibi H, Nasrollahi A, Hedayati M, Taghizadeh M. Effect of flaxseed oil on serum systemic and vascular inflammation markers and oxidative stress in hemodialysis patients: a randomized controlled trial. Int Urology Neph J 2016; 48 (8): 1335- 1341.
49. Haliga RE, Iancu RI, Butcovan D, Mocanu V. Flaxseed prevents leukocyte and platelet adhesion to endothelial cells in experimental atherosclerosis by reducing sVCAM-1 and vWF. Sci World J 2013; 2013: 303950.
50. Hao H, Hongfei M, Shuang R, Li C, Zhilei S, Jiqu X, et al. Flaxseed oil containing flaxseed oil ester of plant sterol attenuates high-fat diet-induced hepatic steatosis in apolipoprotein-E knockout mice. J Functional Foods 2015; 13: 169- 182.
51. Hussein SA, El Senosi YA, Hassanien MR, Hammad MM. Evaluation of the protective role of flaxseed oil on inflammatory mediators, antioxidant defense system and oxidative stress of liver tissue in hypercholesterolemic rats. Int J Pharma Sci 2016; 6 (3): 1480-1489.
52. Wang TM, Chen CJ, Lee TS, Chao HY, Wu WH, Hsieh SC, et al. Docosahexaenoic acid attenuates VCAM-1 expression and NF-kappaB activation in TNF-alpha-treated human aortic endothelial cells. J Nutr Biochem 2011; 22(2): 187-194.

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