Response of mef2 Gene of Slow and Fast Twitch Muscles  of Wistar Male Rats to One Bout of Resistance Exercise

Fathi , M; Gharakhanlou , R; Solimani , M; Rajabi , H

Volume 24, Issue 8 (Nov 2016) JSSU 2016, 24(8): 630-639 | Back to browse issues page

Mendeley

Zotero

RefWorks

Fathi M, Gharakhanlou R, Solimani M, Rajabi H. Response of mef2 Gene of Slow and Fast Twitch Muscles of Wistar Male Rats to One Bout of Resistance Exercise. JSSU 2016; 24 (8) :630-639
URL: http://jssu.ssu.ac.ir/article-1-3697-en.html

Response of mef2 Gene of Slow and Fast Twitch Muscles of Wistar Male Rats to One Bout of Resistance Exercise

M Fathi

, R Gharakhanlou ^*

, M Solimani

, H Rajabi

Abstract: (6657 Views)

Introduction: Myocyte Enhancer Factor 2 (mef2) gene relates with multiple myogenic transcriptional factors that induces activation Muscle-specific genes. MEF2 contributes in muscular cells development and differentiation as well as in fibers transition in response to stimulants. Therefore, the aim of this study was to evaluate the effect of one bout of resistance exercise (RE) on mef2 gene expression in fast and slow skeletal muscles of Wistar male rats.

Methods: For this experimental study, 15 rats from Pasteur Institute were prepared and housed under natural conditions (temperature, light/dark (12:12) cycle, with ad libitum access to food and water) and then randomly divided assigned to RE (n=10) and control groups (n=5); the RE group performed one RE session. 3 and 6 hours following, the rats were anaesthetized and sacrificed, then the soleus and Extensor digitorum longus (EDL) muscles were removed. determine mef2 gene expression rate, the Quantitative Real time RT-PCR was used. Data were analyzed by one sample and independent samples t test.

Results: In EDL muscle, in response to one RE session, the mef2 gene expression increased non significantly at 3 hour (p=0/093) and increased significantly (p=/008) at 6 hour after exercise, but in soleus muscle, the mef2 gene expression decreased significantly at 3 hour (p=0/01), and at 6 hour after RE session there was no observed significant change (p=0.247).

Conclusion: Mef2 expression gene is differently changes in muscle fibers, which are likely associated with changes in fiber type in response to resistance exercise.

Keywords: Muscle Fibers, Fast-Twitch, Muscle Fibers, Slow-Twitch, Myocyte Enhancer Factor 2, Resistance Exercise

Full-Text [PDF 664 kb] (2445 Downloads)

Type of Study: Original article | Subject: Exercise Physiology
Received: 2016/04/21 | Accepted: 2016/09/24 | Published: 2016/12/25

References

1. Fathi M, Gharakhanlou R, Solimani M, Rajabi H, Rezaei R. The effect of resistance exercise on myoD expression in slow and fast muscles of wistar rats. J sport biosci 2015; 6(4): 435-49.

2. Fathi M. Increase of pgc-1 alpha gene expression accompanied by left ventricular structural changes caused by endurance training. J Zabol University Med Scis Health Serv 2015; 7(3): e4238.

3. Black BL, Olson EN. Transcriptional control of muscle development by myocyte enhancer factor-2 (MEF2) proteins. Annu Rev Cell Dev Biol 1998; 14: 167-96.

4. Friday BB, Mitchell PO, Kegley KM, Pavlath GK. Calcineurin initiates skeletal muscle differentiation by activating MEF2 and MyoD. Differentiation; Res Biologic Diversity 2003; 71(3): 217-27.

5. Al-Khalili L, Kotova O, Tsuchida H, Ehren I, Feraille E, Krook A, et al. ERK1/2 mediates insulin stimulation of Na(+),K(+)-ATPase by phosphorylation of the alpha-subunit in human skeletal muscle cells. J Biol Chem 2004; 279(24): 25211-8.

6. McGee SL. Exercise and MEF2-HDAC interactions. Appl Physiol Nutr Me 2007; 32(5): 852-6.

7. Potthoff MJ, Wu H, Arnold MA, Shelton JM, Backs J, McAnally J, et al. Histone deacetylase degradation and MEF2 activation promote the formation of slow-twitch myofibers. J Clin Invest. 2007; 117(9): 2459-67.

8. Lucia A, Burd NA, West DWD, Staples AW, Atherton PJ, Baker JM, et al. Low-Load High Volume Resistance Exercise Stimulates Muscle Protein Synthesis More Than High-Load Low Volume Resistance Exercise in Young Men. PLoS One 2010; 5(8): e12033.

9. Campos G, Luecke T, Wendeln H, Toma K, Hagerman F, Murray T, et al. Muscular adaptations in response to three different resistance-training regimens: specificity of repetition maximum training zones. Euro J Appli Physiol 2002; 88(1-2): 50-60.

10. Dimitrov VG, Arabadzhiev TI, Dimitrova NA, V. DG. Eccentric Contraction-Induced Muscle Fibre Adaptation. Bio Automation 2009;13 (4):119-26.

11. Bassel-Duby R, Olson EN. Signaling Pathways in Skeletal Muscle Remodeling. Annu Rev Biochem 2006; 75: 19-37.

12. McGee SL, Sparling D, Olson AL, Hargreaves M. Exercise increases MEF2- and GEF DNA-binding activity in human skeletal muscle. FASEB J 2006; 20(2): 348-9.

13. Liu Y, Heinichen M, Wirth K, Schmidtbleicher D, Steinacker JM. Response of growth and myogenic factors in human skeletal muscle to strength training. Br J Sports Med 2007; 42(12): 989-93.

14. Raue U, Slivka D, Jemiolo B, Hollon C, Trappe S. Myogenic gene expression at rest and after a bout of resistance exercise in young (18-30 yr) and old (80-89 yr) women. J Appl Physiol 2006; 101(1): 53-9.

15. Wu H, Naya FJ, McKinsey TA, Mercer B, Shelton JM, Chin ER, et al. MEF2 responds to multiple calcium-regulated signals in the control of skeletal muscle fiber type. EMBO J 2000; 19(9): 1963-73.

16. Kadi F. The effects of heavy resistance training and detraining on satellite cells in human skeletal muscles. J Physiol 2004; 558(3): 1005-12.

17. Wu H, Rothermel B, Kanatous S, Rosenberg P, Naya FJ, Shelton JM, et al. Activation of MEF2 by muscle activity is mediated through a calcineurin-dependent pathway. EMBO J 2001; 20(22): 6414-23.

18. Drummond MJ, McCarthy JJ, Fry CS, Esser KA, Rasmussen BB. Aging differentially affects human skeletal muscle microRNA expression at rest and after an anabolic stimulus of resistance exercise and essential amino acids. Am J Physiol Endocrinol Metabolism 2008; 295(6): E1333-40.

19. Livak KJ, Schmittgen TD. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method. Methods 2001;25(4):402-8.

20. Gong H, Xie J, Zhang N, Yao L, Zhang Y. MEF2A binding to the Glut4 promoter occurs via an AMPKalpha2-dependent mechanism. Med Sci Sports Exerc 2011;43(8):1441-50.

21. Cohen TJ, Barrientos T, Hartman ZC, Garvey SM, Cox GA, Yao TP. The deacetylase HDAC4 controls myocyte enhancing factor-2-dependent structural gene expression in response to neural activity. FASEB J 2009; 23(1): 99-106.

22. Koves TR, Li P, An J, Akimoto T, Slentz D, Ilkayeva O, et al. Peroxisome proliferator-activated receptor-gamma co-activator 1alpha-mediated metabolic remodeling of skeletal myocytes mimics exercise training and reverses lipid-induced mitochondrial inefficiency. J Biol Chem 2005; 280(39): 33588-98.

23. McGee SL, Hargreaves M. Exercise and myocyte enhancer factor 2 regulation in human skeletal muscle. Diabetes 2004; 53(5): 1208-14.

24. Czubryt MP, Olson EN. Balancing contractility and energy production: the role of myocyte enhancer factor 2 (MEF2) in cardiac hypertrophy. Recent Prog Horm Res 2004; 59: 105-24.

25. Adams GR, Hather BM, Baldwin KM, Dudley GA. Skeletal muscle myosin heavy chain composition and resistance training. J Appl Physiol 1993;74(2): 911-5.

26. Vissing K, McGee SL, Roepstorff C, Schjerling P, Hargreaves M, Kiens B. Effect of sex differences on human MEF2 regulation during endurance exercise. Am J of physiol Endocrinol Metabol 2008; 294(2): E408-15.