Volume 23, Issue 11 (Feb 2016)                   JSSU 2016, 23(11): 1116-1126 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sadat Razavi Hoseini N, Yari F, Aghaei A. Effects of Platelets on Platelet Concentrate Product on the Activation of Human Peripheral Blood Monocyte Cells. JSSU. 2016; 23 (11) :1116-1126
URL: http://jssu.ssu.ac.ir/article-1-3409-en.html
Abstract:   (4546 Views)

Introduction: Monocytes can interact with platelets due to their surface molecules such as P-selectin glycoprotein ligand-1 (PSGL-1), and form monocyte-platelet complex. In the present study, the effects of platelets interaction of platelet concentrates (PCs) and peripheral blood monocytes were investigated in vitro as a model to predict the probable interactions of these cells and consequently activation of monocytes.

Methods: In this experimental study, units of whole blood and PCs were prepared from Tehran Blood Transfusion Center. After isolation of monocytes from the whole blood, these cells were treated with PC- derived platelets. The activation of monocytes was assessed before and after treatment by the analysis of the respiratory burst of monocytes using dihydrorhodamine 123 (DHR-123). The study data were analyzed using the non-parametric test of Wilcoxon.

Results: The purity of monocytes was determined as 86.1±2 using NycoPrep method. The respiratory burst of monocytes was increased after exposure with platelets. In fact, the difference was significant when platelets were used on the 5th day of storage (P=0.001).

Conclusions: The study findings revealed that platelets have an efficient capacity to stimulate and activate monocytes. The possible involvement of molecules in the interaction of platelet-monocyte demand to be further studied in future.

Full-Text [PDF 647 kb]   (1650 Downloads)    
Type of Study: Original article | Subject: Immunology
Received: 2015/09/16 | Accepted: 2016/01/3 | Published: 2016/03/29

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2022 CC BY-NC 4.0 | SSU_Journals

Designed & Developed by : Yektaweb