Journal of

Introduction: Angiogenesis is regarded as a complex activity required for physiological process as well as many pathological conditions as solid tumor progression and metastasis. Stachys Lavandulifolia of Laminacea family prossesses antioxidant and anti-apoptosis effects. Therefore, this study aimed to investigate the effect of Stachys Lavandulifolia alcoholic extract on angiogenesis in chorioallantoic membrane of Ross chicken embryo.

Methods: In this experimental study, 30 Ross fertilized eggs were divided into 5 random groups consisting of: control, sham-exposed, treatment with 25, 50, 100 mg/kg doses of alcoholic extract. On the 2^nd day of incubation, windows were opened for eggs under the sterile conditions. On the 8^th day, a gelatine sponge with 1×4×4 diameter (Albumen and Agar solution in normal saline) was placed on chorioalantoic membrane (CAM) to which extract was added. On the 12^th day, length and weight as well as chorioallantoic membrane (CAM) of embryos were photographed. Then, the number and length of vessels in a special area on the CAM were measured by Image J. The study results were analyzed by Minitab (16) via applying t-test and ANOVA.

Results: Length and weight mean of embryos as well as number and length of vessels in control and
sham-exposed groups did not show any significant differences. Length and weight mean of embryos and vessels length in all treatment groups demonstrated a significant difference compared to the sham group, Moreover, mean of vessel number did not reveal any significant differences in dose of 50.

Conclusion: As the study findings indicated, the effect of this extract is dose dependet, so as it demonstrates pro angiogenesis in low doses and anti-angiogenesis effect in high doses.