Antioxidant effects of betaine against Indomethacin-induced  gastric damage in rats

Alirezaei , M; Khalighian , N; Shokrani , H; Tanideh, N

Volume 24, Issue 7 (Oct 2016) JSSU 2016, 24(7): 565-575 | Back to browse issues page

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Alirezaei M, Khalighian N, Shokrani H, Tanideh N. Antioxidant effects of betaine against Indomethacin-induced gastric damage in rats. JSSU 2016; 24 (7) :565-575
URL: http://jssu.ssu.ac.ir/article-1-3673-en.html

Antioxidant effects of betaine against Indomethacin-induced gastric damage in rats

M Alirezaei ^*

, N Khalighian

, H Shokrani

, N Tanideh

Abstract: (5864 Views)

Introduction: Betaine (trimethyl glycine) is known as methyl group donor and antioxidant in previous reports. The aim of this study was to assess the antioxidant effects of betaine in Indomethacin-induced gastric damages.

Methods: Thirty-two adult male Sprague–Dawley rats in an experimental study were divided into four equal groups as follow: Control, Indomethacin, Betaine-indomethacin and Ascorbic acid-indomethacin. Control and indomethacin groups received normal saline and betaine and ascorbic acid-pretreated rats were administrated betaine (1.5% of the total diet) and ascorbic acid (50 mg/kg body weight) for 15 consecutive days, respectively. After 24 h fasting, all of the groups received indomethacin (48 mg/kg body weight) and control group received distilled water.

Results: Indomethacin administration increased gastric ulcer occurrence (%) in comparison with control group and betaine pretreatment significantly decreased ulcer occurrence (%) when compared to the other groups (P=0.0017). Gastric wall glutathione peroxidase (GPx) activity was significantly lower in indomethacin group in comparison with the other groups (P=0.0012) while, betaine and ascorbic acid pretreatment increased GPx activity in comparison with indomethacin group (P=0.0012). Catalase activity was significantly higher in betaine-pretreated rats in comparison with indomethacin and ascorbic acid-indomethacin groups (P=0.0015). Lipid peroxidation significantly decreased in betaine and ascorbic acid pretreated groups (P=0.0013).

Conclusion: These results showed beneficial antioxidant effects of betaine against gastric damages induced by indomethacin in rats.

Keywords: Antioxidant Enzymes, Betaine, Gastric Ulcer, Ascorbic Acid

Full-Text [PDF 384 kb] (1664 Downloads)

Type of Study: Original article | Subject: Biochemistry
Received: 2016/04/10 | Accepted: 2016/10/22 | Published: 2016/12/18

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