Volume 25, Issue 12 (Feb 2018)
JSSU 2018, 25(12): 994-1002 |
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Sanjarani F, Esmaeilidehaj M, Zareh-Mehrjardi F, Eghbali T, Ebrahim Rezvani M. The effect of salvigenin on learning, memory and mRNA expression rate of Brain-derived neurotrophic growth factor (BDNF) in maleAlzheimer disease model rat hippocampus
. JSSU 2018; 25 (12) :994-1002
URL: http://jssu.ssu.ac.ir/article-1-4023-en.html
URL: http://jssu.ssu.ac.ir/article-1-4023-en.html
Fatemeh Sanjarani 
, Mansour Esmaeilidehaj , Fatemeh Zareh-Mehrjardi , Tayebe Eghbali , Mohammad Ebrahim Rezvani *
, Mansour Esmaeilidehaj , Fatemeh Zareh-Mehrjardi , Tayebe Eghbali , Mohammad Ebrahim Rezvani *
Abstract: (4697 Views)
Introduction: Effects of many flavonoids have been studied on memory, learning and improvement of Alzheimer. Many flavonoids are effective in the improvement of Alzheimer. Since no study has been conducted on the effect of salvigenin on memory and learning, our aim was to examine the effect of this flavonoid.
Methods: Intraperitoneal injection of D-galactose at a dose of 120 mg / kg was used for 45 for creation of Alzheimer disease model. In this experimental study, 28 male wistar rats weighing approximately 200-250 grams were divided into 4 groups with 7 members, including: normal group: rats that received no drugs, control group: alzheimer disease model rat, which had been stricken to Alzheimer by intraperitoneal injection of D-galactose at a dose of 120 mg/kg and 1, 2 salvigenin groups: in which each group was the same as the control group. Furthermore, they received daily 10 or 20 mg/kg of salvigenin by gavage. In order to evaluate memory, shuttle box and passive avoidance learning was used 2 and 7 days after learning. To assess the mRNA expression rate of BDNF, the entire RNA of hippocampus was isolated and after synthesis of Complementary DNA (cDNA), real time and PCR were done and relative expression of mRNA was evaluated.
Results: The results showed that daily administration of different doses of salvigenin can slow down Alzheimer's induction. The delay duration in entering the dark compartment in trained rats in the treated group was significantly more than the control group. mRNA expression rate of BDNF in salvigenin receiving groups was more than control group.
Conclusion: Generally it can be concluded that salvigenin can improve the memory caused by Alzheimer and also increase mRNA expression rate of BDNF in Alzheimer's rats.
Methods: Intraperitoneal injection of D-galactose at a dose of 120 mg / kg was used for 45 for creation of Alzheimer disease model. In this experimental study, 28 male wistar rats weighing approximately 200-250 grams were divided into 4 groups with 7 members, including: normal group: rats that received no drugs, control group: alzheimer disease model rat, which had been stricken to Alzheimer by intraperitoneal injection of D-galactose at a dose of 120 mg/kg and 1, 2 salvigenin groups: in which each group was the same as the control group. Furthermore, they received daily 10 or 20 mg/kg of salvigenin by gavage. In order to evaluate memory, shuttle box and passive avoidance learning was used 2 and 7 days after learning. To assess the mRNA expression rate of BDNF, the entire RNA of hippocampus was isolated and after synthesis of Complementary DNA (cDNA), real time and PCR were done and relative expression of mRNA was evaluated.
Results: The results showed that daily administration of different doses of salvigenin can slow down Alzheimer's induction. The delay duration in entering the dark compartment in trained rats in the treated group was significantly more than the control group. mRNA expression rate of BDNF in salvigenin receiving groups was more than control group.
Conclusion: Generally it can be concluded that salvigenin can improve the memory caused by Alzheimer and also increase mRNA expression rate of BDNF in Alzheimer's rats.
Type of Study: Original article |
Subject:
Physiology
Received: 2016/12/24 | Accepted: 2018/01/20 | Published: 2018/04/25
Received: 2016/12/24 | Accepted: 2018/01/20 | Published: 2018/04/25
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